ihc accessory kit  (Vector Laboratories)

Journal: Oncogenesis

Article Title: Rab37-mediated OPN secretion enriches SPP1 + macrophages through autocrine–paracrine signaling to drive lung tumor progression

doi: 10.1038/s41389-026-00596-3

Figure Lengend Snippet: A UMAP visualization of macrophage subclusters, showing three distinct populations defined by gene marker expression. B Distribution of macrophage subclusters across Rab37 WT and KO normal and tumor lung tissues. Multiplex IF-IHC staining ( C ) and quantitative analysis ( D ) of orthotopic lung tumor tissues from Rab37 WT and KO mice, showing CD163 (blue) co-localization with OPN (red). Nuclei were counterstained with DAPI (white). Yellow arrows indicate CD163 + / OPN + cells. Scale bar, 20 μm. E , F Representative images of multiplex IF-IHC staining of lung cancer patient samples showing triple labeling of CD163 (blue), Rab37 (green), and OPN (red) in tumor sections (Scale bar, 20 μm), with enlarged views highlighting co-localization. Yellow arrows indicate CD163 + /Rab37 + /OPN + cells, while white arrows denote CD163 + /OPN + cells ( E ). Quantification of CD163 + Rab37 + OPN + tumor-associated macrophages in patients without recurrence and with recurrence ( F ). Kaplan–Meier survival curves comparing overall ( G ) and recurrence-free ( H ) survival of patients stratified by the percentage of OPN + /Rab37 + /CD163 + cells. I Pathway enrichment analysis based on differentially expressed genes in Spp1 + macrophages. Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, Student’s t test.

Article Snippet: Staining was performed using the Opal Multiplex IHC kit (#NEL810001KT, Akoya Biosciences, Marlborough, MA, USA) according to the manufacturer’s instructions.

Techniques: Marker, Expressing, Multiplex Assay, Immunohistochemistry, Labeling